Fig. 5: Plasticity of MS GABAergic neuron responses to reward-predictive cues. | Nature Communications

Fig. 5: Plasticity of MS GABAergic neuron responses to reward-predictive cues.

From: A bottom-up reward pathway mediated by somatostatin neurons in the medial septum complex underlying appetitive learning

Fig. 5

a Optrode recording from MS GABAergic neurons during conditioning when a sound cue (CS) was presented repetitively before sucrose (US) delivery. b Top, raster plot of spikes of an example MS GABAergic neuron in response to tone (upper) and the corresponding peri-stimulus spike time histogram (PSTH) (lower) in a naïve mouse. Inset: average spike waveform of this neuron (shade, s.d.). Scale, 0.5 ms. Red dash line marks the onset of the tone. Bottom, heatmap for responses of all recorded neurons (n = 26, upper) and the population average of Z-score (lower). c, d Similar to (b), but for mice during two days’ training (n = 36 and 31 for day 1 and day 2 respectively, 50 trials per session). Red dash line marks the onset of CS and US, respectively. The heatmap illustrates data from one animal while the lower Z-score plot is the population average across 3 animals. e For mice which had completed two day’s training (n = 17 cells). f Mean Z-score during tone stimulation (0–2 s) in naïve mice, during training (day 1, day 2) and after training (trained). Naïve vs D1, *p = 0.015, D2 vs Trained, p = 0.9046; ****p < 0.0001, one-way ANOVA and post-hoc Tamhane’s T2 multiple comparisons test, two-sided. Source data are provided as a Source Data file.

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