Table 2 Measured POC concentrations in the collected fractions from the axenic P. cf. balticum culture.

From: Mucospheres produced by a mixotrophic protist impact ocean carbon cycling

Fraction

Replicate 1

Replicate 2

Replicate 3

Mean (SD)

C in MilliQ controls (mg L−1)

0.16 (0.04)*

0.16 (0.04)*

0.16 (0.04)*

0.16 (0.04)*

C in spent medium filtrate (mg L−1)

13.54

13.56

12.92

13.34 (0.36)

C in MilliQ 0.4 µm filter control (carbon introduced when removing the mucus from the spent medium filtrate) (mg L−1)

0.84 (0.08)*

0.84 (0.08)*

0.84 (0.08)*

0.84 (0.08)*

C in corrected spent medium filtrate (spent medium filtrate – MilliQ 0.4 µm filter control) (mg L−1)

12.70

12.72

12.08

12.5 (0.36)

C in mucus and spent medium filtrate (mg L−1)

13.39

13.33

12.89

13.20 (0.27)

Calculated C in mucus (mucus and spent medium filtrate – corrected spent medium filtrate) (mg L−1)

0.69

0.61

0.81

0.70 (0.10)

C in cell pellet fraction (mg L−1)

1.20

1.33

1.33

1.29 (0.08)

C in natural seawater control (mg L−1)

0.59 (0.11)*

0.59 (0.11)*

0.59 (0.11)*

0.59 (0.11)*

Calculated C in cell pellet (cell pellet fraction – natural seawater control) (mg L−1)

0.61

0.74

0.73

0.69 (0.07)

Sum of C in all fractions (corrected spent medium filtrate + mucus + cell pellet) (mg L−1)

14.00

14.07

13.62

13.90 (0.24)

C in whole water (mg L−1)

14.59

14.21

13.12

13.97 (0.76)

% Recovery of whole water C (mg L−1)

96%

99%

104%

99.67% (4.04)

Calculated C in mucus in 10 mL sample adjusted for 1:3 dilution (mg 10 mL−1 sample) (multiplied by 0.03)

0.02

0.02

0.02

0.02

Estimated number of mucospheres in 10 mL of sample analysed

131,450

137,140

141,170

136,587 (4,833)

Carbon per mucosphere (mg)

1.6 × 10−7

1.3 × 10−7

1.7 × 10−7

1.53 × 10−7 (0.21 × 10−7)

Carbon per mucosphere (pg)

157.5

133.4

172.1

154.33 (19.54)

  1. Direct measures are shown in white rows; calculated estimates are shown in bold text.
  2. *Mean of 5 replicates.
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