Fig. 5: Liver-derived ARSA does not modulate skeletal muscle lipid rafts. | Nature Communications

Fig. 5: Liver-derived ARSA does not modulate skeletal muscle lipid rafts.

From: Deep proteomic profiling unveils arylsulfatase A as a non-alcoholic steatohepatitis inducible hepatokine and regulator of glycemic control

Fig. 5: Liver-derived ARSA does not modulate skeletal muscle lipid rafts.

a ARSA degrades sulfatides that are primarily localized to cell surface lipid rafts, which are microdomains enriched in cholesterol, sphingolipids, and membrane proteins. b Skeletal muscle lipid raft composition, shown as a percentage of the major lipid classes (average of n = 6/group). CHOL cholesterol, PL phospholipid, TAG triglyceride. c Skeletal muscle sulfatide content in Control-AAV (white bar) and ARSA-AAV (blue bar) mice (n = 6/group). d Volcano plot showing no difference in the proteins contained in skeletal muscle lipid rafts derived from Control-AAV and ARSA-AAV mice. e 1-14C-2-deoxyglucose (2-DG) uptake into differentiated C2C12 myotubes following 24 h incubation without (white bar) or with ARSA (10 ng/mL, blue bar) (n = 3/group, from three individual experiments). Data are means ± SEM. Data assessed using two-tailed unpaired t tests (c, e). Source data are provided as a Source Data file.

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