Fig. 4: Biocompatibility tests of E-GES.

a, b Cell proliferation tests of HACAT (a) and HSF (b) cells in different concentrations of E-GES leaching solutions. Mean ± SD of three independent experiments were shown (n = 3). c, d Viability of HACAT (c) and HSF (d) cells cultured with 10 mg/mL E-GES leaching solution. Live cells and dead cells are illustrated by green colour and red colour, respectively. Scale bar=100 μm. Images shown are representative of three independent experiments (n = 3). e, f Apoptosis tests of HACAT (e) and HSF (f) cells cultured with 10 mg/mL E-GES leaching solution. Images shown are representative of three independent experiments (n = 3). g, h, Photographs of biocompatibility experiments of E-GES on rabbit skin. The skin was treated with E-GES (g) and pretreated artificial sweat (h). The pretreated artificial sweat was prepared by immersing E-GES into the artificial sweat for 24 h. Images shown are representative of four independent experiments (n = 4).