Fig. 1: Ethanol extract of Psoralea corylifolia extends the replicative lifespan of Saccharomyces cerevisiae.

a Viability curve of the haploid MEP strain ZHY1 in liquid with a 10 μg/ml ethanol extract of P.corylifolia or DMSO, the mutation of fob1Δ is known to increase RLS (n = 3). b The RLS was determined by micromanipulating individual yeast cells on YEPD with or without the ethanol extract of P. corylifolia. The mean for WT = 24.7 generations (n = 59); fob1Δ = 30.7 generations (n = 60); and the P. corylifolia ethanol extract at 10 μg/ml = 31.2 generations (n = 59). c Column: MIGHTYSIL RP-18, 5 μm. Eluents: solvent A: 0.1% formic acid—acetonitrile and solvent B: 0.1% formic acid–water. Elution profile: 0–20 min, 60–50% B (40–50% A); 20–35 min, 50–40% B (50–60% A); 35–45 min, 40–30% B (60–70% A); 45–55 min, 30–20% B (70–80% A); 55–60 min, 60–50% B (40–50% A); 70 min, stop. Detection: UV at 245 nm. The RLS was determined by micromanipulating individual yeast cells on YEPD with or without the n-hexane extract of P. corylifolia. d The mean for WT = 23.7 generations (n = 104); fob1Δ = 30.8 generations (n = 78); and the n-hexane extract of P. corylifolia at 10 μg/ml = 29.0 generations (n = 96). e The mean for WT = 23.7 generations (n = 104); sir2Δ fob1Δ double deletion strain (sir2Δ fob1Δ) = 22.9 generations (n = 102); and sir2Δfob1Δ double deletion strain with the n-hexane extract of P. corylifolia at 10 μg/ml (sir2Δ fob1Δ+ n-hexane) = 25.5 generations (n = 102). f The mean for WT = 23.7 generations (n = 104); tor1Δ = 27.5 generations (n = 104); and tor1Δ with the n-hexane extract of P. corylifolia at 10 μg/ml (torΔ+ n-hexane) = 26.9 generations (n = 103). Data presented as mean ± SD from at least three biologically independent experiments. a p values were determined by two-way ANOVA (multiple comparisons). b, d–f p values were determined by the Gehan–Breslow–Wilcoxon test (see also Supplementary Fig. 14). Source data are provided as a Source data file.