Fig. 5: Corylin docked with Gtr1 to inactivate the TOR1 pathway.
From: The flavonoid corylin exhibits lifespan extension properties in mouse
a A docking model of corylin in the N-terminal domain of Gtr1 shown as a ribbon (PDB ID: 3R7W). Ligand–protein interactions with the binding residues of Gtr1 (light gray) and corylin (blue). b Ligand–protein interactions with the binding residues of Gtr1 and corylin. The green dashed lines indicate hydrogen bonds and the pink dashed lines indicate π-interactions. c The peptide sequence for the chemical shift assay. d The Gtr1/Rag A sequence 159–180 in various species. e 1H NMR spectrum of peptide in the presence or absence of corylin. The arrows indicate the upfield chemical shift in the presence of corylin at a molar ratio of corylin and peptide of 1:1. f The RLS was determined by micromanipulating individual yeast cells on YEPD with or without corylin. The mean for WT = 24.3 generations (n = 77); 15 μM corylin = 29.7 generations (n = 75); gtr1Δ = 29.5 generations (n = 78); and gtr1Δ with 15 μM corylin (gtr1Δ+ corylin) = 29.5 generations (n = 78). g The mean for WT = 25 generations (n = 26); 30 μM corylin = 31.6 generations (n = 26); 60 μM corylin = 30.1 generations (n = 26); gtr1Δ = 29.8 generations (n = 26); gtr1Δ+ 30 μM corylin = 29.3 generations (n = 26); gtr1Δ+ 60 μM corylin = 29.7 generations (n = 26). h Immunoblotting was performed using anti-GFP and anti-PGK1 antibodies, and the pGAL-Gtr1 strain was cultured in YEPD and YEPG. i The RLS was determined by micromanipulating individual pGAL-Gtr1 yeast cells on YEPD with or without corylin. The mean for WT = 25.1 generations (n = 51); 15 μM corylin = 30.8 generations (n = 52); pGAL-GTR1 = 28.2 generations (n = 52); and pGAL-Gtr1 with 15 μM corylin (pGAL-Gtr1+ corylin) = 27.8 generations (n = 51). j pGAL-Gtr1 yeast cells were cultured on YEPG to conduct a micromanipulation assay. The mean for WT = 26 generations (n = 47); 15 μM corylin = 29.9 generations (n = 46); pGAL-Gtr1 = 24.7 generations (n = 51); and pGAL-Gtr1 with 15 μM corylin (pGAL-Gtr1 + corylin) = 25.2 generations (n = 51) from at least three biologically independent experiments. f, g, i, j, p values were determined by the Gehan–Breslow–Wilcoxon test (see also Supplementary Fig. 14). Source data are provided as a Source data file.
