Fig. 7: Corylin prolongs lifespan in aged HFD-fed mice.
From: The flavonoid corylin exhibits lifespan extension properties in mouse
Forty-week-old male mice were fed a HFD with or without corylin treatment (n = 30 per group). a Survival curves of mice fed a HFD versus 1%(v/v) corylin (HFD/C) show a significant (Gehan–Breslow–Wilcoxon test χ2 = 4.887 and p = 0.0271) improving lifespan. b, c The bodyweight of mice after started fed with HFD or HFD/C and food consumption (The start point to fed corylin indicated by a black arrow, n = 30 at start point). d Circulating level of corylin (n = 4 biologically independent samples). e Rearing behavior was analyzed in obese mice fed a HFD with or without corylin for 35 weeks under dim and bright light conditions (HFD: n = 6; HFD/C n = 9 biologically independent animals). Latency to falling in the f constant rotarod and (HFD: n = 10; HFD/C: n = 8 biologically independent animals) g accelerating rotarod tests (HFD: n = 7; HFD/C: n = 5 biologically independent animals). h Western of total muscle for Phospho-mTOR Ser2448 (P-mTOR), total-mTOR (T-mTOR) and β-Actin (n = 3). i Quantification was normalized with T-mTOR (n = 3 biologically independent samples). j Fasting blood glucose level result (n = 9 biologically independent samples, p = 0.0058). k Serum biochemical marker results (n = 5 biologically independent samples, total cholesterol p = 0.0037, low-density lipoprotein p = 0.0151, triglyceride p = 0.0486, aspartate transaminase p = 0.0006, creatinine p < 0.0001). Values are expressed as the mean ± SEM. a p values were determined by the Gehan–Breslow–Wilcoxon test. e–g, i–k p values were determined by two-tailed unpaired Student’s t-test. Source data are provided as a source data file.
