Fig. 6: Triple-activation across the vCA1, BLA, and PL5 during post-cond NREM.

a Representative example of triple-activation across vCA1, BLA, and PL5 ensembles. Wideband and filtered LFP recorded from the vCA1, BLA, and PL5 are shown at the top. Instantaneous ensemble activation strength and spikes from the member cells of the ensembles are shown at the bottom. b Representative examples of triple CCGs (top), distribution of triple CCG peak height of the surrogates and the peaks of the actual data (middle), and CCGs of participating ensemble pairs (partial-pairs; bottom). The white hexagons on the top panels indicate the range of peak detection (time gaps of all combinations <100 ms). The leftmost panel illustrates the triplet shown in (a). The inset on the right illustrates the order of ensemble activation. c Distribution of triple CCG peak position (left) and ensemble activation order (right) across coupled-ensemble-triplets. The peak distribution is non-uniform (p < 0.001, χ² test) with a significant peak (**p < 0.01, based on Poisson distribution with Bonferroni correction). The grey line and shaded areas on the right panel indicate the mean and 95% confidence interval, respectively, assuming uniform distribution. ***p < 0.001, **p < 0.01, test based on Poisson distribution with Bonferroni correction. d The proportion of the partial-pairs of coupled-ensemble-triplets with significant peaks on their CCG during post-cond NREM. The numbers of partial-pairs are superimposed on the bars. e Box plots indicating the BLA–vCA1–PL5 triple-activation event occurrence rate per coupled-ensemble-triplet. The centre lines and limits of the boxes represent the median and quartiles, respectively, and whiskers extend to the maxima and minima with outliers excluded. ***p < 0.001, WSR-test. f Oscillatory event peak-triggered average of triple-activation event rates of coupled-ensemble-triplets. The lines and shaded areas indicate the means and SEMs, respectively. Periods with significant differences between pre- and post-cond are indicated with black ticks on the top (p < 0.05, WSR-test). g Triple-activation event-triggered average of wavelet power in the vCA1, BLA, and PL5. The numbers of ensemble triplets analysed are summarised in Supplementary Table 4. Detailed statistics are shown in Supplementary Data 1. Source data are provided as a Source Data file.