Fig. 3: Spatial organisation of RNAPII depends upon myosin VI.

A Example STORM render image of RNAPII-pSer5 under normal, TIP-treated and MVI (MVI) knockdown conditions (scale bar 2 μm). Further widefield example images are shown in Supplementary Figs. 7, 10. B Cluster analysis of RNAPII nuclear organisation under the conditions described in (A). Individual data points correspond to the average value for a cell ROI (n = 14 for normal, 12 for TIP, 13 for KD, 10 for Control siRNA and DMSO). The values represent the mean from the ROIs for each condition (Only statistically significant changes are highlighted ***p < 0.001; ****p < 0.0001 by two-tailed t test compared to normal conditions). C Western-blot against MVI under Normal, MVI-knockdown (MVI siRNA) and TIP-treated conditions. Quantification relative to Normal shows a 95% reduction for MVI siRNA. TIP represents 103% relative to Normal. Example widefield images of MVI under knockdown conditions are shown in Supplementary Fig. 2. D Number of localisations of RNAPII-pSer5 under the different conditions. Individual data points correspond to the value for a cell ROI (n = 14). The values represent the mean from the ROIs for each condition. E Western-blot against RNAPII-pSer5 under normal, TIP-treated and MVI-knockdown (MVI siRNA) conditions. Quantification relative to Normal shows a 5% reduction for TIP. MVI siRNA represents 105% relative to Normal. F RNAPII-pSer5 ChIP against labelled loci under normal and TIP-treated conditions. Values are the average of three independent experiments. Error bars represent SEM from three independent experiments.