Fig. 6: PIEZO2 but not IDR5^del inhibits neurite outgrowth from N2a cells.

a Representative inverted fluorescent images of N2A-P1KO transfected with GFP vector in control medium (left), GFP, PIEZO2 (middle) or IDR5^del (right) in medium without serum and with nerve growth factor (NGF). b Representation of the longest neurite measured (in µm) per cell for GFP in control medium and GFP (gray), PIEZO2 (black) and IDR5^del (blue) in medium without serum and with NGF. Comparison with Kruskal–Wallis test, p < 0.0001, and Dunn’s post-test: GFP control vs. GFP + NGF w/o serum (P < 1e−15), GFP + NGF w/o serum vs. PIEZO2 (P < 1e−15), PIEZO2 vs. IDR5^del (P < 1e−15). Symbols are individual cells, with red line being the median. N-number of cells per group is: GFP control (N = 343), GFP (N = 743), PIEZO2 (N = 733), IDR5^del (N = 685). c Cumulative distribution of the number of neurites per cell across GFP control, GFP, PIEZO2 and IDR5^del group. Total number of cells per group is: GFP control (N = 836), GFP (N = 854), PIEZO2 (N = 998), IDR5^del (N = 810). Source data are provided as a Source Data file.