Fig. 1: Validation of PfRAP01 and PfRAP21 transgenic lines. | Nature Communications

Fig. 1: Validation of PfRAP01 and PfRAP21 transgenic lines.

From: Functional genomics of RAP proteins and their role in mitoribosome regulation in Plasmodium falciparum

Fig. 1

Schematic representation of the TetR-aptamer integration strategy into endogenous pf3d7_0105200 (a) and pf3d7_1470600 (c). TetR-dozi-BSD cassette (black), HA-tag (red), PCR primers, homologous regions used (blue) and recodonized sequences (light blue) are indicated. Genotype analyses of parental, PfRAP01 (b) and PfRAP21 (d) clone lines. PCRs were performed on genomic DNA from indicated lines using different primer combinations. The PCRs are representative of three independent experiments. Immunoblot detection of PfRAP01 (e) and PfRAP21 (f) expression across the asexual blood cycle. The membranes were probed with anti-HA and anti-aldolase was used as loading control. The expression of PfRAP01 and PfRAP21 were normalized by aldolase expression. The blue arrow indicates PfRAP01 degradation. Scale bar: 2 μm. The immunoblots shown are representative of two independent experiments.

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