Fig. 5: CXCR2 ligands function downstream of TBX3 and promote tumor cell proliferation. | Nature Communications

Fig. 5: CXCR2 ligands function downstream of TBX3 and promote tumor cell proliferation.

From: Targeting myeloid derived suppressor cells reverts immune suppression and sensitizes BRAF-mutant papillary thyroid cancer to MAPK inhibitors

Fig. 5

a, b PTC cells were infected with specific shRNA against CXCL1/2 or CXCL8, and subjected to CCK8 (a) or colony formation assay (b). c, d TBX3 knock-down PTC cells with or without CXCLs over-expression were subjected to cell growth assays of CCK8 (c) or colony formation (d). e K1 cells with TBX3 knock-down and Vector or CXCLs over-expression were transplanted into athymic mice subcutaneously. Tumor weights as well as curves were generated and statistically compared, n = 6. f HE and IHC staining of indicated factors were performed on tumor sections in (e). Scale bars, 50μm. n = 3 biological independent samples (a–d). Data are shown as the mean ± s.d. (a–e). P values were calculated by unpaired two-tailed Student’s t test (a–e). Statistical source data are provided in Source Data.

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