Fig. 2: Long-lived hepatic retention and replenishment of Vδ1 and Vɣ9Vδ2-T_RM.

a Representative flow cytometry plot and summary data of total γδ T-cells (as percentage of total CD3⁺ T-cells) in peripheral, hepatic and portal venous blood obtained from cirrhotic patients undergoing transjugular intrahepatic portosystemic shunt procedures (n = 4, p = 0.04). b Representative flow cytometry plot and summary data demonstrating absence of CD69⁺CD49a⁺ or CD69⁺CD103⁺ γδT_RM in peripheral, hepatic, portal venous blood (n = 4 matched samples). c Flow cytometry plot for the identification of donor human leukocyte antigen (HLA) A2⁺ and recipient HLA A2⁻ derived γδ T-cells from two explants obtained 7 years or 11 years following HLA-mismatched liver transplantation. Vδ1 and Vɣ9Vδ2 T-cell frequencies within recipient-derived and donor-derived γδ T-cell subsets. d CD69⁺CD49a⁺ and CD69⁺CD103⁺ expression on donor-derived (HLA A2⁺) and recipient-derived (HLA A2⁻) γδ T-cell subsets (n = 2). Error bars, mean ± SEM (b). Two-tailed p-values were determined by Friedman test with Dunn’s post-hoc test for multiple comparisons (a, b).