Fig. 2: High-resolution Brillouin imaging of ex vivo porcine cornea with a 532 nm wavelength.
From: Measuring mechanical anisotropy of the cornea with Brillouin microscopy
a Schematic showing en face Brillouin imaging of the cornea using a 532 nm laser focused to a spot size of approximately 0.5 μm. b Brillouin image of ex vivo porcine cornea measured en-face, showing relatively uniform Brillouin value across the x–y plane. c Schematic showing Brillouin imaging of the corneal cross-section. The laser was focused just below the surface of the cross-section. d Brillouin images of corneal cross-section at three different (relative) depths, revealing a lamellar pattern. e Transmission electron microscopy (TEM) image of corneal cross-section showing individual lamellae with alternating fibril orientations. A keratocyte is visible in between the two lamellae at the center of the image (keratocyte appears black with substructure). f Higher resolution TEM image of the same region. Individual collagen fibrils can be seen aligned to the preferred directions in two adjacent lamellae.
