Fig. 5: Dbf4 displaces the NSD of Mcm4 on the DH.
From: Structural Insight into the MCM double hexamer activation by Dbf4-Cdc7 kinase
Side views of the cryo-EM density maps of the WT DH before (a) and after (b) DDK phosphorylation and the two mutant DHs, DHΔN140 (c) and DH-ΔN174 (d). The structured fragments of Mcm4-NSD are highlighted in red. e, f Zoomed-in views of (a) and (c) with the atomic model of the DH superimposed, highlighting the structured NSD on the NTD-A of Mcm4. g Zoomed-in view of the cryo-EM map of the DH-DDK (State I) with the atomic model superimposed. The kinase core is removed to highlight the binding of the M4NB and M6NB motifs of Dbf4 to the NTD-As of Mcm4 and Mcm6. Note that the M4NB of Dbf4 and the NSD of Mcm4 share an overlapping binding surface on the NTD-A of Mcm4. h–j Magnified views of the boxed regions in (e–g) respectively.
