Fig. 6: AHR regulates key factors involved in regenerative response.

a Overlap between identified AHR ChIP targets in open chromatin regions with genes up or downregulated in response to FICZ from RNA-sequenced WT organoids (4h-post FICZ treatment). b Functional annotation of activated (red) or repressed (blue) AHR targets using GO ontology analysis (GO biological process) (c) Selection of active AHR targets with their associated functions. Targets that were also identified by the IPA upstream regulator analysis in d4 ENR AHR KO organoids are in bold (see Fig. 2 C). d IgV graphs showing sites of AHR binding in an enhancer element of Cdx2 and and (e) AHR binding to the Sox9 promoter. f qPCR expression data of Cdx2 and Sox9 in WT and AHR KO organoids treated with FICZ (4 h). Statistical significance was determined by two-way ANOVA with Sidak’s multiple comparison test. P value of >0.05 was considered not significant (n.s.). n = 4 and n = 3 mice per genotype were used for Cdx2 and Sox9 qPCRs respectively. Error bars displayed on graphs represent the mean ± SD of at least two independent experiments. Source data for (6a–c, f) are provided with this paper in the source data file.