Fig. 1: Co-enhancement of FAO enzymes and CD47 links to the recurrent GBM patients and poor prognosis.
From: Fatty acid oxidation fuels glioblastoma radioresistance with CD47-mediated immune evasion
a Scored expression of mitochondrial FAO enzymes (CPT1A, CPT2, ACAD9) and CD47 in a group of 46 recurrent high-grade gliomas (HGG) paired with their primary tumor biopsies (n = 46; paired two-tailed t-test). Representative IHC panels shown with scored FAO enzymes and CD47; scale bar = 100 µm). Pearson correlation coefficient analysis of CD47 expression levels with CPT1A (b), CPT2 (c), and ACAD9 (d) in the paired HGG tumors shown in a. Pearson correlation analysis was applied. Pearson correlation coefficient (r) and P values from Pearson correlation tests were shown. e Expression of CPT1A, CPT2, ACAD9, and CD47 in primary versus recurrent tumors from the CGGA database (n = 504; 504 HGG containing 220 recurrent and 284 primary GBM; the recurrent tumors were not individually matched with primary tumors). The box represents the 25th and 75th percentile, lines show medians, and error bars depict 1.5X IQR. An unpaired two-tailed t-test was applied. f Hierarchical clustering of RNA-seq of CD47 with FAO genes in a group of 504 GBM patients from the CGGA database. g Gene set enrichment analysis for a cluster of 157 FAO-related genes in CD47 high or low expressing tumors with green curve indicating a coordinative enrichment score and normalized enrichment score (NES) generated with GSEA software (NES = 1.337798 and P = 0.002). Pearson correlation coefficient analysis of CD47 expression levels with CPT1A (h), CPT2 (i), and ACAD9 (j) in HGG tumors from the CGGA database. Pearson correlation analysis was applied. Pearson correlation coefficient (r) and P values from Pearson correlation tests were shown. Kaplan–Meier overall survival (OS) of patients CGGA database categorized by high (red, n = 252) or low (blue, n = 252) expression of CPT1A (k, P = 0.03), CPT2 (l, P = 8.7E-7), ACAD9 (m, P = 0.2142), and CD47 (n, P = 6.09E-6). Kaplan–Meier survival analysis was applied. Results represent means ± SD. Source data are provided as a Source Data file.
