Fig. 4: A-to-I editing alters the dsRNA structure formed by RCMs within flanking introns of circRNAs.
From: ADARs act as potent regulators of circular transcriptome in cancer
a Predicted secondary structures formed by circCHEK2 RCM with or without A-to-I(G) editing by RNAfold. Partial RNA structures which contain editing sites and neighboring sequences are shown. Blue and red arrows indicate unedited/wildtype (WT) adenosines and edited/mutated (edited) sites, respectively. b Migration on native polyacrylamide gel of RNA probes containing the circCHEK2 RCM sequence with or without A-to-I(G) editing at each editing site. Simplified secondary structure of each probe is shown. Calculation of the relative migration rate was discussed in Methods. Data are presented as the mean ± S.D. of biological triplicates. c, d Predicted secondary structures formed by RCMs of ADAR1/2-promoted circRNAs (circASH1L, circANKLE2-1 and circRNF114) c and ADARs-repressed circRNAs (circRHOT1, circSYNC and circDHX34) d by RNAfold, with or without editing. Partial RNA structures which contain editing sites and neighboring sequences are shown. Blue and red arrows indicate WT adenosines and edited sites, respectively. e Migration on native polyacrylamide gel of RNA probes containing the wildtype (WT) and edited (edt) partial RCM sequences of the indicated circRNAs. Representative result of n  =  2. a, c, d Base-pair probabilities are shown by a color spectrum. Source data are provided in Source Data file.
