Fig. 5: Atg5 deletion in CD11c⁺ cells increases IL-23 induction.

a WT and Atg5 CD11c^KO mice were fed HFD for 14 weeks according to the scheme, n = 6. After 14 weeks of HFD, hepatic CD11c⁺ cells were isolated. b Volcano plot comparison of whole transcriptome gene expression of sorted hepatic WT and Atg5 CD11c^KO cells from mice fed HFD for 14 weeks, n = 6. Differentially expressed genes (p-value < 0.05), with changes of at least 2-fold change (FC) are shown in orange (upregulated) or blue (downregulated). c Network analysis of upregulated (red) and downregulated (green) genes overall significantly predicted to induce liver inflammation. d Heat plot of differentially expressed genes related to IL-23 pathway. e Representative flow cytometric plots of IL-23 expression in hepatic CD11c⁺ cells in WT and Atg5 CD11c^KO mice after 14 weeks of HFD. f Percentage of hepatic IL-23⁺ CD11c⁺ cells after 0, 4, 8, 10, and 14 weeks of HFD, n = 5 mice. g Serum IL-23 levels in WT and Atg5 CD11c^KO after 14 weeks of HFD, n = 8 mice. Error bars are the mean ± SD, two-tailed Student’s t test. Experiments were performed three times. Source data are provided as a Source Data file. Mouse image provided with permission from Servier Medical Art.