Fig. 2: The HitRS signaling system is finely tuned by KrrA through modulating mRNA stability.
From: An RNA-binding protein acts as a major post-transcriptional modulator in Bacillus anthracis
A To examine the effects of krrA deletion on the stability of hitPRS transcripts, northern blot analysis was carried out in WT and ΔkrrA in the presence or absence of ‘205 using a pool of four sequence-specific probes against hitPRS. The housekeeping gene 16 S rRNA serves as a sample loading control. The experiments were performed at least three times and representative images are shown. Source data are provided as a Source Data file. The mRNA stability of hitR (B, C) and gyrA (D, E) was examined in two sets of strains: B. anthracis WT PhitermC and B26 suppressor mutant (B, D), and WT and ΔkrrA (C, E). Relative abundance of hitR (B, C), or gyrA (D, E) was quantified using qPCR. The housekeeping gene gyrA serves as a negative control. The mRNA half-life was determined using a single exponential decay model. Data shown are three biological replicates (mean ± SD). Significant differences are determined by two-tailed t tests.
