Fig. 1: Design principles of the stable coculture system.
From: Design of stable and self-regulated microbial consortia for chemical synthesis
The two strains are engineered to utilize glycerol and glucose, respectively, and cross-feed each other with amino acids and the TCA cycle intermediates. Blue crosses denote gene knockouts to block glucose/glycerol utilization; red crosses denote gene knockouts to block glutamate biosynthesis and the TCA cycle. MMCF multi-metabolite cross-feeding. Genes: glK encodes glucokinase, ptsG encodes PTS glucose transport protein, manXYZ encodes mannose permease, pykA/F encodes pyruvate kinase, ppc encodes phosphoenolpyruvate carboxylase, glpK encodes glycerol kinase, gdhA encodes glutamate dehydrogenase, gltBD encodes glutamate synthase. Metabolites: PEP phosphoenolpyruvate, PYR pyruvate, α-KG α-ketoglutarate, L-GLU L-glutamate, AAs amino acids.
