Fig. 3: Efficient salidroside production and scale-up using coculture engineering.
From: Design of stable and self-regulated microbial consortia for chemical synthesis
a Schematic of the E. coli-E. coli cocultures to accommodate the salidroside biosynthetic pathway and convert a glycerol and glucose mixture to salidroside (The salidroside biosynthetic pathway is shown in orange. For the detailed pathway, see Supplementary Fig. 5). Curves of cell growth and population change in b the Neutralistic Bgly1-Tyr/Bglc1-Sal coculture, c the Commensalistic Bgly1-Tyr/Bglc2-Sal coculture, and d the Mutualistic Bgly2-Tyr/Bglc2-Sal coculture. e Comparison of salidroside titers in the three coculture systems. f Scale-up production of salidroside using the Bgly2-Tyr/Bglc2-Sal coculture. MMCF multi-metabolite cross-feeding, IIRs the initial inoculation ratios. Metabolites: PEP phosphoenolpyruvate, G6P glucose-6-phosphate, UDPG uridine diphosphate glucose, α-KG α-ketoglutarate, L-GLU L-glutamate, AAs amino acids. Data shown are mean ± SD (n = 3 independent experiments). Source data are provided as a Source Data file.
