Fig. 4: Production of coniferol using coculture engineering.
From: Design of stable and self-regulated microbial consortia for chemical synthesis
a Schematic of the E. coli-E. coli cocultures to accommodate the coniferol biosynthetic pathway and convert a glycerol and glucose mixture to coniferol (The coniferol biosynthetic pathway is shown in sapphire. For the detailed pathway, see Supplementary Fig. 5). b Curves of cell growth and population change in the static Bgly2-Caf/Bglc2-Con coculture. c Titers of coniferol and caffeate at 48 h in the Bgly2-Caf/Bglc2-Con coculture. d Curves of cell growth and population change in the dynamic Bgly2-Caf/Bglc2-ConDmpR coculture. e Titers of coniferol and caffeate at 48 h in the Bgly2-Caf/Bglc2-ConDmpR coculture. MMCF multi-metabolite cross-feeding, IIRs the initial inoculation ratios. Genes: gdhA encodes glutamate dehydrogenase, gltBD encodes glutamate synthase, Enzymes: GdhA glutamate dehydrogenase, DmpR the caffeate-responsive transcription factor. Metabolites: PEP phosphoenolpyruvate, α-KG α-ketoglutarate, L-GLU L-glutamate, AAs amino acids. Data shown are mean ± SD (n = 3 independent experiments). Source data are provided as a Source Data file.
