Fig. 1: Acute CNO treatment of adipo-GqD mice causes reduced blood glucose and plasma free acid levels.
From: Adipocyte Gq signaling is a regulator of glucose and lipid homeostasis in mice
a Expression levels of GqD mRNA in different tissues of adipo-GqD (GqD) and control mice (LSL-hM3Dq mice that did not harbor the adipoq-Cre transgene) (n = 4 per group). The presence of GqD transcripts was detected via qRT-PCR. S. Muscle, skeletal muscle; iWAT, inguinal white adipose tissue; eWAT, epididymal white adipose tissue; BAT, brown adipose tissue. b Body weights of adipo-GqD and control mice (mouse age: 12 weeks; n = 8–10 per group). c Blood glucose levels after CNO injection (10 mg/kg i.p.) following a 4 hr fast (n = 7–9 per group). d, e Effect of acute CNO treatment (10 mg/kg i.p.) on glucose tolerance (d, GTT) and insulin tolerance (e, ITT). GTT: 2 g glucose/kg after an overnight fast; ITT: 0.75 U insulin/kg after a 4 hr fast (n = 8 or 9 per group). f, g, Plasma leptin (f), and plasma free fatty acid (FFA) (g) levels of control and adipo-GqD mice 30 min after injection of CNO (10 mg/kg i.p.) following a 4 hr fast (n = 11 or 12 per group). Metabolic tests (c–g) were carried out with male mice that were 10-16 weeks old. Data are presented as means ± s.e.m. b, c (AUC), f, g: two tailed Student’s t-test; c–e: two-way ANOVA followed by Bonferroni’s post hoc test). Source data are provided as a Source data file.
