Fig. 2: Panx1 deletion on endothelial cells mitigates leukocyte trafficking in AAA. | Nature Communications

Fig. 2: Panx1 deletion on endothelial cells mitigates leukocyte trafficking in AAA.

From: Endothelial pannexin-1 channels modulate macrophage and smooth muscle cell activation in abdominal aortic aneurysm formation

Fig. 2

a Comparative histology and immunohistochemistry performed on day 14 indicates a marked decrease in CD3 + T cell, neutrophil (PMN), and macrophage (Mac-2) immunostaining, increase in smooth-muscle-cell α-actin (SMα-actin) expression, and decrease in elastic-fiber disruption in aortic tissue (Verhoeff–Van Gieson, VVG staining for elastin) of elastase-treated EC-Panx1−/− mice compared with elastase-treated WT and SMC-Panx1−/− mice. n = 5/group. Arrows indicate areas of immunostaining. Scale bar is 200 μm. b–f Quantification of immunohistochemical staining demonstrating a significant decrease in neutrophils (PMNs), macrophages, CD3 + T cells, and elastin degradation (VVG) staining, as well as increase in smooth muscle α-actin expression in elastase-treated EC Panx1−/− aortic tissue compared with elastase-treated WT and SMC-Panx1−/− mice. No significant differences were observed between elastase-treated WT and SMC-Panx1−/− mice. *P < 0.0001 vs. WT control; **P < 0.0001 vs. WT elastase and SMC-Panx1−/−; #P = 0.0003 vs. WT elastase and SMC-Panx1−/−. Data is represented as mean values ± SEM and comparative statistical analyses were done by one-way ANOVA.

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