Fig. 3: Pharmacological inhibition of Panx1 mitigates AAA formation in the elastase-treatment model.

a Schematic depicting treatment protocol for probenecid (PBN) in the elastase model of AAA. b PBN treatment attenuates aortic diameter in elastase-treated male WT mice compared with elastase treatment alone. *P = 0.0001 vs. all other groups; n = 14/group. c Representative images of aortic phenotype in the respective groups. d–i Comparative histology displayed a marked decrease in leukocyte infiltration and elastin-fiber disruption, as well as increase in SMα-actin expression in PBN-treated mice compared with elastase-treated mice alone.*P = 0.007; **P = 0.01; n = 5/group. Arrows indicate areas of immunostaining. Scale bar is 400 μm. j–n Pro-inflammatory cytokine expression in aortic tissue is significantly attenuated in elastase-treated WT mice after PBN administration compared with elastase-treated mice alone. *P < 0.0001 vs. respective controls; ^#P < 0.001 vs. elastase.; n = 6/group. o A significant decrease in aortic tissue ATP content was observed in PBN-treated mice compared with elastase-treated mice alone. *P < 0.0001 vs. controls; ^#P < 0.0001 vs. elastase; n = 8 mice/group. p Expression of MMP2 in aortic tissue was significantly attenuated in PBN-treated mice compared with elastase-treated mice alone. *P < 0.0001 vs. control; ^#P = 0.0003 vs. elastase; n = 5/group. Data are represented as mean values ± SEM and comparative statistical analyses were done by one-way ANOVA followed by multiple comparisons. Source data are provided as a Source Data file.