Fig. 4: Sequence variations modulate interactions between natural CidA^wPip and CidB^wPip alleles.

a Sequence alignment shows that residues at certain positions are different among the CidA^wPip and CidB^wPip variants. The residues that are the same among the variants are not shown. The varied residues which are located at the binding interfaces are boxed. The numbers above sequence alignment were based on the sequences of CidA and CidB from wPip Tunis. b The locations of the varied residues are shown as spheres on the structure of the CidA^wPip(Tunis)-CidB^wPip(Tunis)_ND1-ND2 complex. c The varied residues which are located at the binding interfaces are colored and labeled. Other residues at the binding interfaces are shown in gray. d, e His-tagged CidA variants were used to pull down GST-tagged CidB variants. The proteins were detected by Coomassie Blue staining. These experiments were repeated three times independently with similar results obtained. One representative is shown. ND nuclease domain. Source data for panels (d) and (e) are provided as a Source Data file.