Fig. 3: Cryptochrome depletion partially restores behavioural and molecular synchronisation during constant light and temperature cycles in s-tim flies.
From: A natural timeless polymorphism allowing circadian clock synchronization in “white nights”
a Group actograms of one representative experiment as described in the legend for Fig. 1a. N (ls-tim; cry02): 17, (s-tim; cry02): 20. b Median of normalised activity of independent experiments combined during day 6 (left panel) of LLTC and day two of LL after LLTC (right panel). Yellow bar: thermophase, blue bar: cryophase (12 h each). N for left panel: (ls-tim; cry02): 76; (s-tim; cry02): 96. N for right panel: (ls-tim; cry02): 36; (s-tim;cry02): 54. Averages of normalised TIM (c) and PER (d) levels in the different groups of clock neurons during day six of LLTC in s-tim; cry02 flies. Number of brain hemispheres/time point = 3–5. Error bars = sem (standard error of the mean). A Mann–Whitney test was performed (with a Bonferroni correction) to compare the CRY-negative with the CRY-positive LNd and the 5th s-LNv at ZT0 and ZT12 (TIM) and ZT18 (PER). Because the PER level was lower in the l-LNv and the s-LNv compared to the other lateral neurons, we also performed a Mann–Whitney test to compare ZT6 with the other time points. * p < 0.05, ** p < 0.01, *** p < 0.0001. Source data are provided as a Source Data file.
