Fig. 4: The visual system phases the locomotor output to the evening without affecting the molecular clock in s-tim;cry0 background.

a Group actograms of one representative experiment as described in the legend for Fig. 1a. N (s-tim, GMR-hid; cry⁰¹): 17, (norpA^P41; s-tim; cry⁰²): 19. b Median of normalised activity of independent experiments combined during day 6 (left panel) of LLTC and day two of LL after LLTC (right panel). Yellow bar: thermophase, blue bar: cryophase (12 h each). N for left/right panel (s-tim, cry⁰²): 56/45, (norpA^P41; s-tim; cry⁰²): 49/40, (GMR-hid, s-tim; cry⁰¹): 32/30. c Averages of normalised TIM (left) and PER (right) levels in the different groups of clock neurons during day six of LLTC in norpA^p41;s-tim;cry⁰² flies. Number of brain hemispheres/time point = 2–5. Error bars = sem (standard error of the mean). A Mann–Whitney test was performed (with a Bonferroni correction) to compare the CRY-negative with the CRY-positive LNd and the 5th s-LNv at ZT0 and ZT12 (TIM), or ZT18 (PER). Note that at ZT12 the TIM level in the CRY-negative LNd is not significantly different from the 5th (p = 0.08). Because the PER level was lower in the l-LNv compared to the other lateral neurons, we also performed a Mann–Whitney test to compare ZT18 with the other time points. * p < 0.05, ** p < 0.01, *** p < 0.0001. Source data are provided as a Source Data file.