Fig. 2: Xmas reporters allow detection of X chromosome expression in mESCs and iPSCs.
From: BAF complex-mediated chromatin relaxation is required for establishment of X chromosome inactivation
a Schematic showing the strategy for reprogramming and analysis of Xmas MEFs. b Flow cytometry data from primary female Xmas MEFs during the reprogramming process (n = 4 independent replicates). c Schematic of the breeding strategy to produce Xmas mESCs, their XCI status in vivo and during culture and differentiation in vitro. Extraembryonic (Ex), Inner Cell Mass (ICM). d Live fluorescent image of XHprt-GFP/XHprt-mCherry Xmas female blastocysts. e Live fluorescent image of cultured mESCs from the indicated genotypes carrying different combinations of the fluorescent reporter alleles. Images shown are from a single experiment. f Flow cytometry of cultured mESCs from the indicated genotypes carrying different combinations of the fluorescent reporter alleles. Source data are provided as a source data file.
