Fig. 4: Xmas mESCs detect impaired XCI during differentiation.
From: BAF complex-mediated chromatin relaxation is required for establishment of X chromosome inactivation
a Flow cytometry data showing the kinetics of the fluorescent reporter alleles during differentiation and XCI of Xmas mESCs for multiple cell lines (n = 9 independent replicates). The triangle represents weaning from 2i to differentiation media in 25% increments over 3 days. b RNA-seq timecourse data from differentiating XFVBXCAST mESCs showing the ratio of XFVB gene expression compared with the XCAST (XFVB—XCAST log2). Hprt expression is represented as a red line and other X-linked genes as grey shade. c–f Flow cytometry data showing the kinetics of the fluorescent reporter allele expression changes during differentiation and XCI of Xmas mESCs. Cells were challenged with shRNAs against the indicated known regulators of XCI or control (Nons) either prior to differentiation depicted as either raw data (c) or normalised to Nons (d), or during differentiation as either raw data (e) or normalised to Nons (f). Triangles represent weaning from 2i media into differentiation media and arrows indicate the day of shRNA viral transduction. n = 3–6 independent replicates from two independent shRNAs per gene, error bars indicate s.e.m., two-way ANOVA, p value is given for the entire timecourse of that gene knockdown. Source data are provided as a source data file.
