Fig. 9: Expansion of crypts with enterocytes expressing Isg15 upon whipworm infection.
From: Defining the early stages of intestinal colonisation by whipworms
Representative images of expression of Isg15 (green) and Krt20 (magenta), visualised by mRNA ISH by HCR, in the caecum of a uninfected mice and b T. muris-infected mice after 72 h of infection. Dashed white lines show the extent of “islands” of Isg15+ crypts. c The number of Isg15+ crypts in a caecal section was calculated as a percentage of the total number of crypts across three sections from the same mouse, and with three mice analysed per condition (uninfected, 24 and 72 h p.i.). Data are presented as median values with interquartile range. **p = 0.0045, ***p = 0.0002 Kruskal Wallis test and Dunn’s comparisons among groups. For each condition, dots representing technical replicates are coloured identically. Source data are provided as a Source data file. d In some instances, worms were located near islands of Isg15+ enterocytes, e while in other cases, worms were found away from these islands. Scale bars: a/ai and b/bi = 60 μm; d/di and e/ei = 30 μm.
