Fig. 4: Evaluation of in vivo immune response of PLX-NP-P-aPD-1@Gel in B16F10 tumor recurrence model. | Nature Communications

Fig. 4: Evaluation of in vivo immune response of PLX-NP-P-aPD-1@Gel in B16F10 tumor recurrence model.

From: Depletion of tumor associated macrophages enhances local and systemic platelet-mediated anti-PD-1 delivery for post-surgery tumor recurrence treatment

Fig. 4

a Representative confocal microscopy images of platelets and the formation of platelet-derived microparticles at the tumor site (n = 3 biologically independent samples). Scale bar, 100 µm. b–e Quantitative analysis of the number of F4/80+ macrophages (b, PLX-NP-P-aPD-1@Gel vs. PLX-NP@Gel: nsP = 0.7842; PLX-NP-P-aPD-1@Gel vs. PLX-NP+P-aPD-1: **P = 0.0026), CD3+ T cells (c, PLX-NP-P-aPD-1@Gel vs. PLX-NP@Gel: ***P = 0.0002; PLX-NP-P-aPD-1@Gel vs. P-aPD-1@Gel: ***P = 0.0009; PLX-NP-P-aPD-1@Gel vs. PLX-NP+P-aPD-1: *P = 0.0100), CD8+ T cells (d, PLX-NP-P-aPD-1@Gel vs. PLX-NP@Gel: ***P = 0.0004; PLX-NP-P-aPD-1@Gel vs. P-aPD-1@Gel: **P = 0.0015; PLX-NP-P-aPD-1@Gel vs. PLX-NP+P-aPD-1: **P = 0.0049), and Granzyme B+ CD8+ T cells (e, PLX-NP-P-aPD-1@Gel vs. PLX-NP@Gel: ***P = 0.0002; PLX-NP-P-aPD-1@Gel vs. P-aPD-1@Gel: **P = 0.0012; PLX-NP-P-aPD-1@Gel vs. PLX-NP+P-aPD-1: **P = 0.0023) per tumor mass in the recurrent tumor tissues after treatments with saline, NP-P@Gel, PLX-aPD-1@Gel, PLX-NP@Gel, P-aPD-1@Gel, PLX-NP+P-aPD-1, and PLX-NP-P-aPD-1@Gel. Data are presented as mean ± s.d. (n = 4 biologically independent samples) and analyzed with one-way ANOVA followed by Dunnett’s multiple comparison test. f IFNγ levels in the tumors detected by enzyme-linked immunosorbent assay (ELISA) after different treatments (PLX-NP-P-aPD-1@Gel vs. PLX-NP@Gel: **P = 0.0056; PLX-NP-P-aPD-1@Gel vs. P-aPD-1@Gel: *P = 0.0197; PLX-NP-P-aPD-1@Gel vs. PLX-NP+P-aPD-1: *P = 0.0199). Data are presented as mean ± s.d. (n = 4 biologically independent samples) and analyzed with one-way ANOVA followed by Dunnett’s multiple comparison test. g Representative confocal microscopy images of immune-stained CD8+ T cells and TAMs in the saline group and PLX-NP-P-aPD-1@Gel group (n = 3 biologically independent samples). Green: TAMs. Red: CD8+ T cells. Blue: Nucleus. Scale bar, 100 µm. The experiments were repeated three times. Source data are provided as a Source Data file.

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