Fig. 2: Heterogeneous DNA methylation alterations in CVID S-mem B cells. | Nature Communications

Fig. 2: Heterogeneous DNA methylation alterations in CVID S-mem B cells.

From: Single-cell Atlas of common variable immunodeficiency shows germinal center-associated epigenetic dysregulation in B-cell responses

Fig. 2

a Dot plot showing principal component analysis based on the DMRs at the single-cell level of naïve and S-mem B cells in the control and CVID twins. The elliptical shadows correspond to the confidence interval (90% CI) for each subpopulation. b Heatmap representing the DNA methylation percentage of the 300 most variable CVID.no-demeth DMRs at the single-cell level. Dark blue samples correspond to S-mem B cells from the control twin; light blue samples correspond to S-mem B cells from the CVID twin. c Plot showing the number of altered hypomethylated DMRs for each individual S-mem B cell for the entire set of cells analyzed at the single-cell level in the CVID twin. d Heatmap and histogram depicting CTCF binding signal in human primary B cells at CVID.no-demeth DMRs ± 2.5 Kb flanking regions. CTCF binding data was obtained from publicly-available ChIP-seq dataset (see Methods). e Heatmap representing the log2(FC) enrichment for several TF binding motifs at the single-cell level over the altered hypomethylated DMRs in S-mem B cells from the CVID twin. Source data for panels in this figure are provided in the Source Data Fig. 2 file.

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