Fig. 1: Two cell-type CRISPR/Cas9 screen using NK cell-mediated killing of GSCs. | Nature Communications

Fig. 1: Two cell-type CRISPR/Cas9 screen using NK cell-mediated killing of GSCs.

From: CHMP2A regulates tumor sensitivity to natural killer cell-mediated cytotoxicity

Fig. 1: Two cell-type CRISPR/Cas9 screen using NK cell-mediated killing of GSCs.

a Schematic of screen design. Screening hits in GSC/NK cell co-culture (n = 2 samples per cell line) were compared with GSC monoculture to identify genes that induce resistance or sensitivity to NK cell killing (created with Biorender.com). b Heatmap of individual and averaged scores of top 25 genes that increase sensitivity or resistance across four GSC models. c GO gene set enrichment analysis of hits. Y axis indicates −log10 FDR of gene set enrichment. d Scatter plot showing the stratification of genes identified by the GSC CRISPR/Cas9 two cell-type screen ordered by score. Highlighted in red are known genes known that typically activate NK cells (MICA, MICB, ULBP1, ULBP2, ULBP3, FAS, TNFRSF10A [TRAIL-R1], TNFRSF10B [TRAIL-R2], and ICAM1; and highlighted in blue are genes are known to inhibit NK cell-mediated activity (HLA-A, HLA-B, HLA-C, HLA-E, TAP1, and TAP2).

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