Fig. 4: Secretion of CXCL10 and CXCL12 from CHMP2A-KO tumor cells increases NK cell migration.

a ELISA comparing CXCL10 concentration WT and KO in CW468 and Cal27 cells. The error bars represent ±SEM across n = 3 independent experiments two-tailed Student’s t test was performed to determine statistical significance. b ELISA comparing CXCL10 concentration in WT and KO in CW468 and Cal27 cells. Error bars represent ±SEM from the mean across n = 3 independent experiments two-tailed Student’s t test was performed to determine statistical significance. c charts representing the number of NK cells migrating towards CW468-WT and KO (top chart) and Cal27-WT and KO (lower chart) cells analyzed by flow cytometry. Error bars represent ±SEM across n = 3 independent experiments. Two-tailed Student’s t test was performed to determine statistical significance. d Immunoblot analysis of NF-κB P65 in Cal27 CHMP2A-WT or KO. GAPDH has been used as a loading control. e Dual-luciferase reporter assay showing NF-κB activity in Cal27 CHMP2A-WT and KO cells. Error bars represent ±SEM from the mean across n = 3 replicates, two-tailed Student’s t test was performed to determine statistical significance. d, e Representative of n = 3 independent experiments.