Fig. 3: Decrease in oxidative capacity in Sirt6 KO mice at basal condition.

a Representative transmission electron micrograph of gastrocnemius muscles (GAS) from WT and Sirt6 KO mice. b Numbers of mitochondria with normal or abnormal morphology were counted from images in (a) (n = 4). c Mitochondrial DNA (mtDNA) was quantified by qPCR using nuclear DNA (nDNA) as a standard (n = 5 for WT, n = 4 KO). d qPCR analysis of genes related to mitochondrial biogenesis and oxidative phosphorylation (OxPhos) in GAS (n = 6). Expression of each gene was normalized with housekeeping Gapdh whereas expression of mitochondrial genome-encoded genes Mtco1 and Mtco2 was normalized with 16S rRNA. e Representative Western blot analysis of OxPhos complex (n = 6). f Oxygen consumption rate (OCR) was measured using Seahorse XF analyzer in myofibers isolated from GAS. Basal respiration, respiration related to ATP production (uncoupled, difference between OCR before and after oligomycin injection), and maximal respiration (difference between OCR after FCCP and antimycin A (AA)/rotenone injection) were determined (n = 15 for WT, n = 12 for KO). Values are mean ± SEM. Data are representative of at least three independent experiments. Unpaired two-tailed t test between two groups was conducted for statistical analyses (b–f). *p < 0.05 and **p < 0.01. Source data are provided as a Source Data file.