Fig. 2: ComFC supports translocation of tDNA across the cytoplasmic membrane.
From: ComFC mediates transport and handling of single-stranded DNA during natural transformation
a Percentage of cells with fluorescent DNA foci for the indicated H. pylori strains. Bars correspond to the average and standard deviation from independent biological experiments. b Time course of DNA foci presence for indicated H. pylori strains. Z maximum projections of merged images of ATTO-550 (red channel) and differential interference contrast (DIC) are presented. Scale bars: 2.5 μm. c and e Stability of DNA foci displayed by H. pylori strains. Data points correspond to the mean and standard deviation from independent experiments (n = 2 for wild-type, n = 3 for comFC, n = 1 for comEC, n = 5 for comFC-T165A and n = 2 for comFC C15S C18S. d Internalisation kinetics of fluorescent DNA in indicated H. pylori strains. GFP expressing bacteria displaying fluorescent DNA foci were followed for 3 h by confocal microscopy in live conditions (Supplementary Movies 1–3). The mean ± SEM for volumes of DNA internalised were measured by 3D-analysis of individual bacterial cells for wild-type (n = 26), ΔcomEC (n = 25), ΔcomFC (n = 148) strains. At least two independent experiments were performed for each strain except for ΔcomEC. P-values calculated using Kruskal–Wallis statistics indicate that ΔcomEC (p < 0.0001) and ΔcomFC (p = 0.0003) curves are significantly different from the wild-type curve. Source data are provided as a Source Data file.
