Fig. 3: ComFC is a membrane-associated protein.
From: ComFC mediates transport and handling of single-stranded DNA during natural transformation

a and b Western blot analysis of subcellular fractions. Localisation of ComFC in wild-type H. pylori strain using an anti-ComFC antibody. Blots are representative of two fractionation experiments. c and d Localisation of overexpressed ComFC-FLAG wild-type and mutant proteins using an anti-FLAG antibody. Blots are representative of 7 and 9 experiments for panels c and d, respectively. The inner membrane protein MotB was used as marker for the fractionation experiments. T: total extract, S: soluble fraction, M: membrane. B: boiled samples, NB: not boiled samples. Source data are provided as a Source Data file.