Fig. 2: Decanoic acid damage stabilization of pHi of S. cerevisiae.
From: Enhancing biofuels production by engineering the actin cytoskeleton in Saccharomyces cerevisiae
a Internalization of FM4-64 under 0 mM and 0.2 mM decanoic acid treatment. Cells were incubated with FM4-64 on ice, then the cells of different conditions were released and incubated at 30 oC. The fluorescence images were shown for timepoints of 0, 2, 4, 6 min at 30 oC. The red arrows point toward the endosome and the white one toward the vacuole. The red arrows point to the endosome and the white arrows point to the vacuolar. b The internalization of fluorescent unit Cy5 conjugated α-factor in 0 mM and 0.2 mM decanoic acid conditions. The cells were incubated with α-factor at 30 oC and then the ratio endocytosis process was dependent on the internalization of Cy5 conjugated α-factor. The data were analyzed by FACS. Significance (p-value) was evaluated by two-sided t-test. c The comparison of in vivo and in vitro pH values in 0 mM and 0.2 mM decanoic acid treatment. d The fluorescence microscope images of S. cerevisiae expression Sup35-GFP fusion protein (a protein that just aggregate under low pHi) under 0 mM and 0.2 mM decanoic acid treatment. Green arrows point toward Sup35 aggregations. Abbreviation: C10 decanoic acid. All the experiments were performed in three biological repeats. Values and error bars represent the mean values and standard deviations of biological repeats. Source data are provided as a Source Data file.
