Fig. 1: First genetic toolkit for Mycoplasma.
From: A genetic toolkit and gene switches to limit Mycoplasma growth for biosafety applications
a Schematic representation of the inducible systems based on the AraR repressor (l-arabinose inducer), LacI repressor (IPTG inducer), CI repressor, TetR repressor (ATc inducer). Blunt arrows indicate repression. b Induction time-course results for one synthetic promoter selected for each repressor system. The upper graphs show synchronised growth (growth index as the ratio Abs430 nm/Abs560 nm) between uninduced (blue) or induced (red and orange) cultures with the corresponding inducer for each repressor. The lower graphs show the corresponding mCherry kinetics (fluorescence in arbitrary units; background subtracted for autofluorescence—see Methods). The mCherry expression depending on the inducer concentration was confirmed by Fluorimetry (c) and western blot (d) in the exponential phase (two days after induction) for several synthetic promoters in each repressor system. All results from mean values from three bio-replicates (black triangles show the values of each bio-replicates). Error bars indicate standard deviation. Source data are provided as a Source Data file.
