Table 1 Strains used in this study.

From: Impact of horizontal gene transfer on emergence and stability of cooperative virulence in Salmonella Typhimurium

Strain name	Strain number	Relevant genotype^a	Resistance^b	Reference
SL1344	SB300	Wild type	Sm	⁶⁵
ATCC 14028S	14028S	Wild type	None	⁶⁶
E. coli CC118 λpir	–	λpir; used for R6K ori replication (e.g. in pKD3)	None	^67,68
SB300 ΔhilD	M3101	ΔhilD	Sm	¹¹
SB300 ΔhilD pVir^Low	Z2325	ΔhilD pVir^Low	Sm, Cm	This work
SB300 ΔhilD pVir^High	Z2326	ΔhilD pVir^High	Sm, Cm	This work
14028S ΔhilD ΔinvG ΔssaV pM975	Z2327	14028S ΔhilD ΔinvG ΔssaV pM975	Amp	This work
Low cost pVir donor	Z2317	14028S ΔhilD ΔinvG ΔssaV pVir^Low pM975	Amp, Cm	This work
High cost pVir donor	Z2236	14028S ΔhilD ΔinvG ΔssaV pVir^High pM975	Amp, Cm	This work
Control donor	Z2318	14028S ΔhilD ΔinvG ΔssaV P2^cat pM975	Amp, Cm	This work
Cheater recipient	Z2235	14028S ΔhilD ssaV::aphT pM975	Amp, Kan	This work
Wild-type pM972	Z2319	14028S ssaV::aphT pM972	Amp, Kan	This work
ΔhilD recipient pM972	Z2320	14028S ΔhilD ssaV::aphT pM972	Amp, Kan	This work
14028S ΔhilD ΔssaV pM975	T144	14028S ΔhilD ΔssaV pM975	Amp	This work
14028S ΔhilD ΔssaV pM975 pVir^Low	T1	14028S ΔhilD ΔssaV pVir^Low pM975	Amp, Cm	This work
Cheater recipient P2	T154	14028S ΔhilD ΔssaV P2^aphT pM975	Amp, Kan	This work
ΔhilD pVir^Low transconjugant pM972 −1	Z2321	14028S ΔhilD ssaV::aphT pM972 pVir^Low	Amp, Kan, Cm	This work
ΔhilD pVir^Low transconjugant pM972 −2	Z2322	14028S ΔhilD ssaV::aphT pM972 pVir^Low	Amp, Kan, Cm	This work
ΔhilD pVir^High transconjugant pM972 −1	Z2323	14028S ΔhilD ssaV::aphT pM972 pVir^High	Amp, Kan, Cm	This work
ΔhilD pVir^High transconjugant pM972 −3	T292	14028S ΔhilD ssaV::aphT pM972 pVir^High	Amp, Kan, Cm	This work
ΔhilD pVir^High transconjugant pM972 −4	T293	14028S ΔhilD ssaV::aphT pM972 pVir^High	Amp, Kan, Cm	This work
ΔhilD pVir^High transconjugant pM972 −5	T294	14028S ΔhilD ssaV::aphT pM972 pVir^High	Amp, Kan, Cm	This work
ΔhilD pVir^High transconjugant pM972 −6	T295	14028S ΔhilD ssaV::aphT pM972 pVir^High	Amp, Kan, Cm	This work
ΔhilD pVir^High transconjugant pM972 −7	T296	14028S ΔhilD ssaV::aphT pM972 pVir^High	Amp, Kan, Cm	This work
Evolved transconjugant Low cost TTSS-1⁺ −1	Z2296	14028S ΔhilD ssaV::aphT pM975 pVir^Low	Amp, Kan, Cm	This work
Evolved transconjugant Low cost TTSS-1⁺ −2	Z2306	14028S ΔhilD ssaV::aphT pM975 pVir^Low	Amp, Kan, Cm	This work
Evolved transconjugant Low cost TTSS-1⁺ −3	Z2310	14028S ΔhilD ssaV::aphT pM975 pVir^Low	Amp, Kan, Cm	This work
Evolved transconjugant Low cost TTSS-1⁺ −4	Z2299	14028S ΔhilD ssaV::aphT pM975 pVir^Low	Amp, Kan, Cm	This work
Evolved transconjugant Low cost TTSS-1⁺ −5	Z2302	14028S ΔhilD ssaV::aphT pM975 pVir^Low	Amp, Kan, Cm	This work
Evolved transconjugant Low cost TTSS-1⁺ −6	Z2308	14028S ΔhilD ssaV::aphT pM975 pVir^Low	Amp, Kan, Cm	This work
Evolved transconjugant Low cost TTSS-1^- −1	Z2298	14028S ΔhilD ssaV::aphT pM975 pVir^Low	Amp, Kan, Cm	This work
Evolved transconjugant Low cost TTSS-1^- −2	Z2305	14028S ΔhilD ssaV::aphT pM975 pVir^Low	Amp, Kan, Cm	This work
Evolved transconjugant Low cost TTSS-1^- −3	Z2301	14028S ΔhilD ssaV::aphT pM975 pVir^Low	Amp, Kan, Cm	This work
Evolved transconjugant Low cost TTSS-1^- −4	Z2304	14028S ΔhilD ssaV::aphT pM975 pVir^Low	Amp, Kan, Cm	This work
Evolved transconjugant Low cost TTSS-1^- −5	Z2309	14028S ΔhilD ssaV::aphT pM975 pVir^Low	Amp, Kan, Cm	This work
Evolved transconjugant High cost TTSS-1⁺ −1	Z2238	14028S ΔhilD ssaV::aphT pM975 pVir^High	Amp, Kan, Cm	This work
Evolved transconjugant High cost TTSS-1⁺ −2	Z2253	14028S ΔhilD ssaV::aphT pM975 pVir^High	Amp, Kan, Cm	This work
Evolved transconjugant High cost TTSS-1⁺ −3	Z2246	14028S ΔhilD ssaV::aphT pM975 pVir^High	Amp, Kan, Cm	This work
Evolved transconjugant High cost TTSS-1⁺ −4	Z2242	14028S ΔhilD ssaV::aphT pM975 pVir^High	Amp, Kan, Cm	This work
Evolved transconjugant High cost TTSS-1⁺ −5	Z2244	14028S ΔhilD ssaV::aphT pM975 pVir^High	Amp, Kan, Cm	This work
Evolved transconjugant High cost TTSS-1⁺ −6	Z2312	14028S ΔhilD ssaV::aphT pM975 pVir^High	Amp, Kan, Cm	This work
Evolved transconjugant High cost TTSS-1⁻ −1	Z2239	14028S ΔhilD ssaV::aphT pM975 pVir^High	Amp, Kan, Cm	This work
Evolved transconjugant High cost TTSS-1⁻ −2	Z2243	14028S ΔhilD ssaV::aphT pM975 pVir^High	Amp, Kan, Cm	This work
Evolved transconjugant High cost TTSS-1⁻ −3	Z2311	14028S ΔhilD ssaV::aphT pM975 pVir^High	Amp, Kan, Cm	This work
Evolved transconjugant High cost TTSS-1⁻ −4	Z2245	14028S ΔhilD ssaV::aphT pM975 pVir^High	Amp, Kan, Cm	This work
Evolved transconjugant High cost TTSS-1⁻ −5	Z2247	14028S ΔhilD ssaV::aphT pM975 pVir^High	Amp, Kan, Cm	This work
Evolved transconjugant High cost TTSS-1⁻ −6	Z2252	14028S ΔhilD ssaV::aphT pM975 pVir^High	Amp, Kan, Cm	This work
Evolved transconjugant High cost TTSS-1⁻ −7	Z2254	14028S ΔhilD ssaV::aphT pM975 pVir^High	Amp, Kan, Cm	This work
Evolved transconjugant High cost TTSS-1⁻ −8	Z2255	14028S ΔhilD ssaV::aphT pM975 pVir^High	Amp, Kan, Cm	This work

^aFor additional information on genotypes of select strains, see Tables S1–4 for a whole-genome resequencing summary.
^bRelevant resistances only: Sm = ≥50 µg/ml streptomycin; Cm = ≥15 µg/ml chloramphenicol; Kan = ≥50 µg/ml kanamycin; Amp = ≥100 µg/ml ampicillin.

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Table 1 Strains used in this study.

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