Table 3 Primers used in this study.
Primer name | Sequence (5′ to 3′) | Purpose | Ref. |
|---|---|---|---|
HilD-31-F | GGAACACTTAACGGCTGACATGGGAATTAGCCATGGTCCATACAGGATAAGCAATTCACCG | Gibson Assembly of hilD into pKD3 (pVirLow) | This work |
HilD-1-F | GGAACACTTAACGGCTGACATGGGAATTAGCCATGGTCCATAGCAGATTACCGCACAGGA | Gibson Assembly of hilD into pKD3 (pVirHigh) | This work |
HilD-2-R | AGAATAGGAACTTCGGAATAGGAACT AAGGAGGATATTCATAGTGTTAATGC GCAGTCTGA | Gibson Assembly of hilD into pKD3 (both pVirLow and pVirHigh) | This work |
HilD-3Po-F | AGGAACTTCGGAATAGGAAC | Verification of hilD in pKD3 | This work |
HilD-4Po-R | AACACTTAACGGCTGACATG | Verification of hilD in pKD3 | This work |
HilD-32-F | GCATGATAATAATAATCAATAACAATAAGCTGTGTCACGTTTACATCATCAGGATAAGCAATTCACCG | λ-red for hilD-cat in P2 downstream of cib (pVirLow) | This work |
HilD-29-F | GCATGATAATAATAATCAATAACAATAAGCTGTGTCACGTTTACATCATGCAGATTACCGCACAGGA | λ-red for hilD-cat in P2 downstream of cib (pVirHigh) | This work |
HilD-30-R | AAGGGTAATGGCGGAAGCCGGATACCCAGCCGCCAGAGAATGTGTAGGCTGGAGCTGCTTC | λ-red for hilD-cat in P2 downstream of cib (both pVirLow and pVirHigh) | This work |
insert_ p2_up | GTA CCG GTG CGT GAT AAC | Verification of hilD-cat insert in P2 to create pVir (both pVirLow and pVirHigh) | |
insert_ p2_dw | CAA CAG CGT GAC CTG CC | Verification of hilD-cat insert in P2 to create pVir (both pVirLow and pVirHigh) | |
ver_hilD_up2 | TCTCGATAGCAGCAGATTAC | Verification of ΔhilD in the chromosome | |
ver_hilD_dw2 | CAGTATAAGCTGTCTTCCG | Verification of ΔhilD in the chromosome | |
ssaV-137F | GCAGCGTTCCAGGGTATTCC | Verification of ΔssaV in the chromosome | This work |
ssaV + 155R | CAGCAAGTTCTTCTCCAGGC | Verification of ΔssaV in the chromosome | This work |
invG-134F | GAAGGCCACGAGAACATCAC | Verification of ΔinvG in the chromosome | This work |
invG + 112R | GCGGCCTGTTGTATTTCCGC | Verification of ΔinvG in the chromosome | This work |
