Fig. 3: Loss of Chd7 in preosteoblasts leads to low bone mass and high marrow adiposity.
From: CHD7 regulates bone-fat balance by suppressing PPAR-γ signaling
a Representative microCT images of distal femurs and midshaft cortical bone from Sp7-cre and Sp7-cre;Chd7fl/fl mice at 4 weeks old (Scale bar, 500 μm). b Quantitative microCT analyses of the distal end of the femurs from Sp7-cre and Sp7-cre;Chd7fl/fl mice at 4 weeks old (n = 8). c Von Kossa staining of undecalcified sections of femurs from Sp7-cre and Sp7-cre;Chd7fl/fl mice at 4 weeks old (Scale bar, 500 μm). d TRAP staining of femur sections from Sp7-cre and Sp7-cre;Chd7fl/fl mice at 4 weeks old (Scale bar, 50 μm). e Dynamic osteogenic index of trabecular bone from the femoral metaphysis in Sp7-cre and Prx1-cre;Chd7fl/fl mice at 4 weeks old, including MAR and BFR determined by double labeling (n = 8). Histomorphological analysis of trabecular bone from the femoral metaphysis in Sp7-cre and Prx1-cre;Chd7fl/fl mice at 4 weeks old, including osteoblast and osteoclast numbers (n = 8). f Representative images of adipocytes in the distal femur marrow in Sp7-cre and Prx1-cre;Chd7fl/fl mice at 4 weeks old. Blacks arrows indicate trabecular bones, and orange arrows indicate marrow adipose tissues (Scale bar, 50 μm). g Quantitative measurements of adipocytes, including number and area of adipocytes in the distal marrow per tissue area. Quantitative data were obtained using the ImageJ software (n = 8). Data are shown as the mean ± S.D.; p value by two-tailed Student’s t test.
