Fig. 5: CHD7 suppresses the transactivation of PPAR-γ.

a GSEA showing decreased enrichment of osteogenesis-related genes and increased enrichment of PPAR-regulated genes in the Chd7-deficient MSCs under osteogenic conditions. b Heatmap of representative osteogenesis-associated genes in the Chd7-deficient MSCs under osteogenic conditions. c GSEA showing increased enrichment of adipogenesis-related genes and PPAR-regulated genes in the Chd7-deficient MSCs under adipogenic conditions. d Heatmap of representative PPAR signaling pathway-associated genes in the Chd7-deficient MSCs under adipogenic conditions. e Immunoprecipitation assay in MSCs from the Chd7^fl/fl and Prx1-cre;Chd7^fl/fl mice. Cell extracts were immunoprecipitated with anti-PPAR-γ antibodies. Immunoprecipitates were detected by western blotting with the indicated antibodies. f Representative immunofluorescence staining of PPAR-γ and H3K4me3 in in MSCs from the Chd7^fl/fl and Prx1-cre;Chd7^fl/fl mice (Scale bar, 20 μm). g Quantification of (f), showing the proportion of H3K4me3⁺ PPAR-γ⁺ nuclei among the total PPAR-γ⁺ nuclei (n = 8). h ChIP-seq results indicating that H3K4me3 and PPAR-γ coaggregate near the promoter region of Adipoq by WashU EpiGenome Browser. i ChIP-qPCR showing that knockout of Chd7 increases the occupancy of H3K4me3 in the promoter regions of Adipoq (n = 3). j ChIP-qPCR showing that knockout of Chd7 increases the occupancy of PPAR-γ in the promoter regions of Adipoq (n = 3). Data are shown as the mean ± S.D.; p value by two-tailed Student’s t test.