Fig. 6: PPAR-γ inhibition partially rescues the phenotype of Chd7-deficient MSCs.
From: CHD7 regulates bone-fat balance by suppressing PPAR-γ signaling
a Western blot analysis and quantification of CHD7, RUNX2, alkaline phosphatase, and PPAR-γ in wild type and Chd7-deficient bone marrow MSCs treated with vehicle or a PPAR-γ inhibitor (GW9662). b Representative images of ALP and ARS staining of treated MSCs. c Quantitative analyses of the ALP activity in treated MSCs (n = 6 biologically independent MSC samples). d Quantitative analyses of the mineralization in treated MSCs (n = 6 biologically independent MSC samples). e qRT-PCR analyses of the mRNA expression of Dlx5, Runx2, and Sp7 in treated MSCs under osteogenic conditions (n = 3 biologically independent MSC samples). f Representative images and quantitative analyses of Nile red staining in treated MSCs. Scale bar, 50 μm (n = 3 biologically independent MSC slides). g qRT-PCR analyses of the mRNA expression of Cebpa, Pparg, and Adipoq in treated MSCs under adipogenic conditions (n = 3 biologically independent MSC samples). Data are shown as the mean ± S.D.; p value by one-way ANOVA with Tukey’s post hoc tests for multiple comparisons.
