Fig. 7: Administration of a PPAR-γ inhibitor partially rescues the skeletal phenotype of Prx1-cre;Chd7fl/fl mice.
From: CHD7 regulates bone-fat balance by suppressing PPAR-γ signaling
a Representative images of the Chd7fl/fl and Prx1-cre;Chd7fl/fl mice at 4 weeks old, treated with vehicle or a PPAR-γ inhibitor (GW9662) (n = 8). b Length of the treated Chd7fl/fl and Prx1-cre;Chd7fl/fl mice at 4 weeks old (n = 8). c Weight of the treated Chd7fl/fl and Prx1-cre;Chd7fl/fl mice at 4 weeks old (n = 8). d Representative microCT images of distal femurs and midshaft cortical bone from the treated Chd7fl/fl and Prx1-cre;Chd7fl/fl mice at 4 weeks old (Scale bar, 500 μm). e Quantitative microCT analyses of the distal end of the femurs from the treated Chd7fl/fl and Prx1-cre;Chd7fl/fl mice at 4 weeks old (n = 8). f Von Kossa staining of trabecular bone from the femoral metaphysis in the treated Chd7fl/fl and Prx1-cre;Chd7fl/fl mice at 4 weeks old (Scale bar, 500 μm). g Histomorphological analysis and dynamic osteogenic index of trabecular bone from the femoral metaphysis in the treated Chd7fl/fl and Prx1-cre;Chd7fl/fl mice at 4 weeks old, including BFR, osteoblast, and osteoclast numbers (n = 8). h Representative images and quantitative measurements of adipocytes in the distal femur marrow in the treated Chd7fl/fl and Prx1-cre;Chd7fl/fl mice at 4 weeks old. Blacks arrows indicate trabecular bones, and orange arrows indicate marrow adipose tissues (Scale bar, 50 μm). i Quantitative data were obtained using the ImageJ software, including the number and area of adipocytes in the distal marrow per tissue area (n = 8). Data are shown as the mean ± S.D.; p value by one-way ANOVA with Tukey’s post hoc tests for multiple comparisons.
