Fig. 3: Human pancreatic M-E17 and M-E20 cell transcriptomes are jointly enriched in secreted factors that have their receptors present in hESC-derived EPs.
a Principal component analysis of human primary M-E cells and control cell transcriptomes showing that pancreatic M-E cells are distinct from other HDFs and HUVECs. b Functional gene annotation of significantly enriched genes upregulated in both M-E17 and M-E20 (fold change >20). c Signaling pathways represented by unique genes significantly upregulated in both M-E17 and M-E20 (fold change >20). d Heatmap of normalized log2 Z-score expression of ECM, secreted growth factors and signal proteins enriched in human M-E17 and M-E20 cells compared to HDFs and HUVECs. Fisher’s exact test was used to estimate p values. e Ligand-receptor connectome analysis based on data from FANTOM5 database and RNA-Seq. Lines connect ligands expressed by M-E cells, control cells, with their receptors expressed in hESC-PPs. Lines’ thickness correlates with the number of such ligand-receptor pairs between cell types. f Graph presenting ligands expressed by M-E cells that have their known receptors expressed in hESC-derived PPs.
