Fig. 4: CCR2b co-expressing B7-H3.CAR-T cells have comparable antitumor activity when coculture with NSCLC cell lines in vitro. | Nature Communications

Fig. 4: CCR2b co-expressing B7-H3.CAR-T cells have comparable antitumor activity when coculture with NSCLC cell lines in vitro.

From: Targeting brain lesions of non-small cell lung cancer by enhancing CCL2-mediated CAR-T cell migration

Fig. 4

a Representative flow plots of CARs expression in CD19.28, B7-H3.28, B7-H3.BB, CCR2b.B7-H3.28 and CCR2b.B7-H3.BB CAR-T cells as assessed by flow cytometry. b, c Summary of the percentage (b) and MFI (c) of CAR expression in B7-H3.28, B7-H3.BB, CCR2b.B7-H3.28 and CCR2b.B7-H3.BB CAR-T cells (n = 10 independent experiments using CAR-T cells generated from ten different donors, *p = 0.0219 for CD19.28 vs. B7-H3.28, *p = 0.0240 for CD19.28 vs. B7-H3.BB, *p = 0.0295 for B7-H3.BB vs. CCR2b.B7-H3.28, *p = 0.0217 for B7-H3.BB vs. CCR2b.B7-H3.BB, one-way ANOVA). Data are presented as mean values ± SD. d, e GFP-labeled NSCLC cell lines were cocultured with CD19.28, B7-H3.28, B7-H3.BB, CCR2b.B7-H3.28 or CCR2b.B7-H3.BB CAR-T cells at the CAR-T cell to tumor cell ratio of 1:5. On day 5, NSCLC cells (GFP+) and CAR-T cells (CD3+) were enumerated by flow cytometry. Representative flow-cytometry plots (d) and quantification of residual tumor cells (e) are illustrated. Data are presented as mean values + SD. f, g Summary of IFNγ (f) and IL2 (g) released by CAR-T cells in the culture supernatant after 24 h of coculture with the indicated cell lines as measured by ELISA, n = 4 independent experiments using CAR-T cells generated from four different donors in (d–g). Data are presented as mean values + SD. Source data for (b, c, e, f, g) are provided as a Source Data file.

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