Fig. 5: Lack of neurogenesis in KIC tumors and sympathetic ganglia.

a–f Sections through the pancreas of 6-week-old KIC mice double-labeled with anti-TH and anti-insulin antibodies. Images show TH⁺/insulin⁺ β-cells in an intact islet (a–c) and scattered inside the tumor (d–f). g–i PDAC sections from 6-week-old KIC mice triple-labeled with anti-TH, anti-DCX, and anti-CD45 antibodies. The inset in (g) shows TH staining of an axon as a positive control. Images are representative of three mice analyzed. j, k Representative 3D view of the coeliac-superior mesenteric ganglia (c.s.m.g.) of 8-week-old control (j) and KIC (k) mice after labeling with anti-TH (red) and ganglia volume reconstruction (white). l Quantification of the volume of the coeliac-superior mesenteric ganglion complex in control and KIC mice. Data are presented as mean ± SEM. n = 3 mice/group. P = 0.08 (Mann–Whitney test). m–r Visualization of TH⁺ neurons and EDU⁺ cells in the coeliac-superior mesenteric complex (c.s.m.g.) of 5.5-week-old control (m–o) or KIC (p–r) mice. No EDU⁺ cells expressed TH. s Quantification of the number of EDU⁺ cells per surface area. Data are presented as mean ± SEM. WT: n = 3 mice, 37 sections and 273 EDU⁺ cells; KIC: n = 3 mice, 34 sections and 265 EDU⁺ cells. P = 0.9407 (Mann–Whitney test). Scale bars = 20 µm (a–i and m–r) and 400 μm (j–k). Source data are provided as a Source Data file.